Composite

Part:BBa_K3738012

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)


MS2 Bacteriophage Maturation Protein A with C-Terminal Histidine Tag (Composite Part)

Maturation Protein A is required in order for the capsid of the MS2 phage, and subsequently MS2-derived phage-like particles (PLP), to bind to bacterial pili. It is released from the capsid upon binding, and enters the cell along with the cargo to be delivered by the MS2 capsid. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, maturation protein A coding region with a C-terminal histidine tag (BBa_K3738002), and double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 881
    Illegal XhoI site found at 964
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 970


[edit]
Categories
Parameters
None